Interferon (IFN)-gamma as a recombinant
pharmaceutics, being launched in Iran as
the third country in the world, despite its
unclear genotoxic potential in in vivo
models. In the present study, the genotoxic
effects of this recombinant human IFNgamma
were evaluated in peripheral blood
lymphocytes from 24 healthy volunteers.
The induction of chromosomal alterations
including chromosomal aberrations, and
micronuclei (MN) in binucleated cells were
performed by different cytogenetic assays.
Heparinized whole-blood samples from
volunteers after cell pretreatment were
treated with 0.33-66.6 IU/μl of IFN-gamma.
The treated cells were then determined for
chromosomal aberrations, and micronuclei in
binucleated cells. The negative and positive
controls were phosphate buffered saline
(PBS), and mitomycin C, respectively.
Results revealed that cells treated with IFN
showed no significant difference from cells
treated with PBS indicating this recombinant
protein under the performed treatment
regimen lacked of genotoxic potential.
JAASP Volume 1 No. 2
April - June, 2012