Medicinal plants have played an important role in human healthcare as the essential ingredients of traditional medicines for thousands of years. Botanical gardens worldwide play a crucial role in the conservation, cultivation and utilization of plant species, and research activities related to ecology, taxonomy, systematics, biotechnology, etc. Medicinal plant gardens have played a significant role in education and research of plant species used for medicinal purposes. Botanical gardens and medicinal plant gardens are also actively involved in organizing of educational programs/events to students and the general public. This article will provide an overview of the educational and research activities of Medicinal Plant Garden at the School of Pharmacy, Kumamoto University, Japan.